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Quantitative Haemagglutination
Author(s) -
Greenwalt T. J.,
Steane E. A.
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01732.x
Subject(s) - sialic acid , autoanalyzer , neuraminidase , hemagglutination , chemistry , microbiology and biotechnology , biochemistry , antibody , biology , chromatography , immunology , enzyme
S ummary . As sialic acid is removed from red cells by neuraminidase treatment agglutinability measured by the sensitive AutoAnalyzer continuous flow technique increases with anti‐A, ‐B, ‐H, ‐D and ‐c. The increase in agglutinability is related to the amount of sialic acid removed, but the relationship is different for different antibodies. Rather than a simple non‐specific reduction in zeta potential, these results suggest a more complex explanation, probably involving the decrease of ordered water near the surface of the desialated cells.