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Studies on the Defective Haematopoietic Microenvironment of Sl/Sl d Mice
Author(s) -
Fried Walter,
Chamberlin William,
Knospe William H.,
Husseini Salah,
Trobaugh Frank E.
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01690.x
Subject(s) - haematopoiesis , spleen , stromal cell , bone marrow , colony forming unit , biology , microbiology and biotechnology , femur , stem cell , immunology , andrology , pathology , cancer research , medicine , genetics , paleontology , bacteria
S ummary . Femurs and spleens of both Sl/Sl d and +/+ origin were implanted into both Sl/Sl d and +/+ hosts. Eight weeks later the +/+ grafts incorporated more radioactive iron than did the Sl/Sl d ones. The number of colony forming units (CFU) in the marrow of +/+ femur grafts and in +/+ spleen grafts was several fold greater than that in Sl/Sl d grafts. X‐irradiation of femur and spleen donors with 950 R reduced the number of CFU in the grafts to 40–60%, whereas grafts from donors exposed to 2500 R supported no CFU growth at all. The haematopoietic cells in the grafts after 6 weeks were almost entirely of host origin. These studies suggest that Sl/Sl d mice have a congenital defect in some stromal elements of their haematopoietic tissues which are essential for the normal growth of CFU. These stromal factors are likely to be cellular, are less radiosensitive than are the CFU, and support the growth of CFU rather than actually differentiate into these cells. These stromal factors are present in both spleens and femurs of mice. The model described here appears to be a useful one for further characterizing stromal factors essential for supporting haematopoiesis.