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Biochemical Changes in the Platelet Energy Transferring System during Concomitant Action of Cephalin and Serotonin
Author(s) -
Pǎuşescu Exacustodian,
Chirvasie Rodica
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01686.x
Subject(s) - glutathione , nad+ kinase , glutathione reductase , nicotinamide , chemistry , biochemistry , platelet , nucleotide , cofactor , serotonin , enzyme , biology , glutathione peroxidase , receptor , gene , immunology
S ummary . The paper concerns the investigations of the changes in the concentrations of endogenous nicotinamide adenine nucleotides (NAD + , NADH, NADP + and NADPH) and reduced glutathione (GSH) during the incubation of canine blood platelets with cephalin and/or serotonin. Activity of NADH‐dependent glutathione reductase in this instance was also determined. These investigations have revealed the following findings: (1) platelets maintained constant levels of nicotinamide adenine nucleotides and GSH during incubation in Hanks’ solution; (2) 5‐HT induced a marked immediate increase in the amounts of NAD + , NADH and GSH and a moderate reduction in the amount of NADPH; no significant change was seen in the amount of NADP + or in the activity of NADH‐dependent glutathione reductase; (3) cephalin had similar effects on NAD + , NADH and GSH, but to a lesser extent; in addition, it induced a significant decrease in the amounts of NADP + and NADPH and a marked activation of NADH‐dependent glutathione reductase; (4) cephalin in addition to 5‐HT amplified these modifications, except for GSH increase (despite an important cephalin‐induced activation of NADH‐dependent glutathione reductase) and produced a marked fall of NADP + and NADPH. These findings suggest that: (a) platelet nicotinamide adenine nucleotides and GSH exhibit a high sensitivity to these different substances (a phosphatide and an amine) with implications for platelet function and aggregation; (b) cephalin and 5‐HT, despite their different chemical nature, act somewhat similarly on these compounds.