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Folated Deficiency in Pernicious Anaemia Measured by Determination of Decreased Serine Synthesis in Lymphocytes
Author(s) -
Ellegaard Jøgen,
Esmann Viggo
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01683.x
Subject(s) - intracellular , serine , pernicious anaemia , vitamin b12 , methionine synthase , methionine , vitamin , medicine , cyanocobalamin , chemistry , endocrinology , biochemistry , phosphorylation , amino acid
S ummary . A functional folate deficiency was determined in four of five patients with untreated pernicious anaemia by a new method (Ellegaard & Esmann, 1972a) for determination of intracellular folate activity utilizing the folate‐dependent incorporation of 14 C‐formate into serine of lymphocytes under standardized conditions. The decreased folate activity of lymphocytes was improved within 3 days of treatment with vitamin B 12 . These observations support the theory of an intracellular, functionally inert pool of 5‐methyl‐tetrahydrofolate in vitamin B 12 deficiency due to impaired methionine synthesis.