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The Detection of Factor‐VIII‐like Antigen in Haemophilic Carriers and in Patients with Raised Levels of Biologically Active Factor VIII
Author(s) -
Denson K. W. E.
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01671.x
Subject(s) - antigen , haemophilia a , antibody , haemophilia , immunology , medicine , chemistry , microbiology and biotechnology , biology , surgery
S ummary . A rabbit antibody has been prepared against therapcutic factor VIII of intermediate purity. Using this antibody in quantitative immunoelectrophoresis, the results of Zimmerman et al (1971a, b) have been confirmed. Female carriers of haemophilia were classified with greater certainty than by the use of bioassay alone. The factor‐VIII‐like antigen was present in all haemophilic patients tested, and was reduced or absent in patients with von Willebrand's disease. The antigen was increased in all cases following physical exercise, the infusion of adrenaline and following major gynaecological surgery. Raised values of factor‐VIII‐like antigen correlated well with increase in biological activity of factor VIII. The method has been applied to whole plasma and the antigen may be quantitated directly without the necessity for initial ethanol fractionation of plasma.