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Polysomes of Cultured Normal and Leukaemic Lymphocytes: Response to Phytohaemagglutinin
Author(s) -
Rabinowitz Y.,
Justice P.,
Wong Polly,
Hsia D. Y. Y.
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01659.x
Subject(s) - polysome , phytohaemagglutinin , stimulation , microbiology and biotechnology , biology , lymphocyte , biochemistry , amino acid , chemistry , ribosome , immunology , endocrinology , rna , gene
S ummary . Polysome OD 260 profiles of glass column separated normal and leukaemic lymphocytes were studied. Contaminating RBC were haemolysed with NH 4 Cl prior to culture. Post‐mitochondrial supernatants from lymphocytes ruptured by nitrogen cavitation were analysed in a scaled‐down sucrose gradient procedure. Normal lymphocytes responded to phytohaemagglutinin stimulation in a few hours with increases in all classes of polysome OD 260 absorbances from the low levels found in both normal and leukaemic unstimulated lymphocytes. Leukaemic lymphocytes which responded to PHA showed delays in both polysome formation and blast‐cell production. With leukaemic lymphocytes the degree of responsiveness to PHA and the amount of polysome OD 260 produced were inversely related to the level of the peripheral WBC counts. Incorporation of [ 14 C]‐amino acids at polysomes in both intact cells and in cell free reactions increased with PHA stimulation and blast‐cell production. With both normal and leukaemic lymphocytes increased [ 14 C]amino acid incorporation was related to increase in polysome OD 260 . There was no evidence that leukaemic polysomes when formed were defective.