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Uptake of 57 Co Vitamin B 12 by Human Haemopoietic Cells in Vitro
Author(s) -
Wickramasinghe S. N.,
Carmel R.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb08877.x
Subject(s) - bone marrow , eosinophil , basophilic , biology , granulocyte , vitamin b12 , vitamin , microbiology and biotechnology , in vitro , lymphoblast , immunology , endocrinology , cell culture , pathology , biochemistry , medicine , asthma , genetics
Summary. Uptake of 57 Co‐B 12 by haemopoietic cells was studied in short‐term cultures of human bone marrow, using autoradiography and scintillation counting. Autoradiographs from vitamin B 12 deficient marrow cultures showed that neutrophil myelocytes, metamyelocytes and mature leucocytes took up the vitamin. No labelling was detected in the eosinophil leucocyte series, in megakaryocytes or in polychromatic megaloblasts. In the erythropoietic series, uptake was seen in some basophilic megaloblasts but only in the most severely megaloblastic marrow, providing direct confirmation that immature erythroblasts take up vitamin B 12 to a much greater extent than the more mature erythroblasts. Cells from normoblastic marrow showed no labelling except for one instance in which supraphysiological amounts of 57 Co‐B 12 were used, when uptake by myelopoietic cells occurred. Scintillation counting of marrow suspensions confirmed the greater uptake by vitamin B 12 deficient than by normoblastic cells. Uptake by vitamin B 12 deficient cells required serum, but there was no difference between vitamin B 12 deficient serum and normal serum.