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The Response of W/W v and Sl/Sl d Anaemic Mice to Haemopoietic Stimuli
Author(s) -
Harrison D. E.,
Russell E. S.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb08797.x
Subject(s) - phenylhydrazine , erythropoiesis , spleen , erythropoietin , medicine , endocrinology , ineffective erythropoiesis , hypoxia (environmental) , bone marrow , chemistry , iron isotopes , red cell , andrology , anemia , microbiology and biotechnology , immunology , biology , oxygen , isotope , physics , organic chemistry , quantum mechanics , medicinal chemistry
Summary: The percentage of haem‐containing nucleated RBC precursor cells in marrows and spleens of W/W v and Sl/Sl d anaemic mice increased in response to hypoxia produced by reduced pressure, red‐cell loss, or red‐cell destruction by phenylhydrazine. The response in the spleens of W/W v mice occurred several days later than the marrow response, in contrast to normal or Sl/Sl d mice in which the marrow and spleen responses occurred simultaneously. These responses were also simultaneous in W/W v mice whose anaemia had been cured by an injection of normal stem cells. After RBC destruction by phenylhydrazine the duration and degree of tissue hypoxia necessary to stimulate the Sl/Sl d response varied widely among individual Sl/Sl d mice. In Sl/Sl d mice with anaemias alleviated by implants of intact normal spleens, most erythropoiesis was contained within these normal spleen grafts. Polycythaemic W/W v and Sl/Sl d anaemic mice gave defective responses to erythropoietin; the defect was not caused by delayed erythroid maturation. Serum samples from anaemic mice responding to RBC destruction by phenylhydrazine contained high concentrations of erythropoietin in quantities inversely proportional to the donor's PCV (packed cell volume), but did not contain any factors, other than erythropoietin, to stimulate erythropoiesis in polycythaemic Sl/Sl d anaemic mice.