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Plasmin Lysis of Fibrinogen and Fibrin and the Antigenic Properties of their Degradation Products
Author(s) -
Ekert H.,
Muntz R. H.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb08791.x
Subject(s) - lysis , plasmin , fibrin , fibrinogen , chemistry , microbiology and biotechnology , agglutination (biology) , biochemistry , immunoelectrophoresis , antibody , chromatography , immunology , biology , enzyme
Summary. Purified fibrinogen was lysed by plasmin and the degradation products identified by gel filtration, immunoelectrophoresis and the tanned red cell haem‐agglutination inhibition (TRCHI) test. After 15 min of lysis, fractions with molecular weight (mol wt) 270 000 (fragment X), 110 000 (containing both fragments D and E) and 15 000 were present. After 6 hr lysis, only fractions of mol wt 80 000 (fragments D and E) and 15 000 were present. Fibrin was lysed by plasmin for 30 min, 4, 8 and 24 hr. In each case fractions of mol wt 150 000 (fragment Y), 80 000 (fragments D and E) and 15 000 were produced. It was shown that early and late products of fibrin lysis are similar to those of fibrinogen, and that the tanned red cell haemagglutination inhibition test using anti‐fibrinogen antibody provides a reliable estimate of fibrin lysis both in vitro and in vivo .