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The Effects of Certain Blood‐Grouping Sera on the Red‐Cell Surface as Seen by the Scanning Electron Microscope
Author(s) -
Longster G. H.,
Tovey L. A. D.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb07099.x
Subject(s) - antiserum , serology , enzyme , cell , red cell , scanning electron microscope , red blood cell , morphology (biology) , electron microscope , microbiology and biotechnology , antigen , chemistry , biology , antibody , biochemistry , immunology , medicine , materials science , genetics , physics , optics , composite material
S ummary . The effect of various blood‐grouping antisera on the surface of red cells either possessing or lacking the appropriate antigens were observed using the scanning electron microscope. Certain of these sera produced non‐specific effects on the cell surface and possible causes of these changes were investigated. It was shown that slight contamination of grouping sera with bacteria or chemicals can result in the sera producing similar effects on the red‐cell morphology. The changes produced by proteolytic enzymes used in blood‐group serology on the red‐cell surface were also examined. Each enzyme was shown to have a different effect on red‐cell morphology, but as all the enzymes apparently have the same serological action, it is concluded that the effect of these enzymes on red‐cell morphology and their serological action are not related.