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Red Cell Survival Studies in Haemoglobin H Disease using [ 51 Cr] Chromate and [ 32 P]Di‐isopropyl Phosphofluoridate
Author(s) -
Tso S. C.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb07097.x
Subject(s) - chromate conversion coating , red cell , chemistry , isotopes of chromium , isopropyl , radiochemistry , red blood cell , nuclear chemistry , cell , medicine , microbiology and biotechnology , biochemistry , biology , chromium , medicinal chemistry , organic chemistry
S ummary . Red‐cell survival was studied in eight patients with haemoglobin H disease in the steady state, using simultaneously [ 51 Cr]chromate and [ 32 P]di‐iso‐propyl phosphofluoridate (DF 32 P). A shortened survival apparently due to a process of random destruction was observed in all patients, and the rate of 51 Cr elution calculated from the DF 32 P data was within the normal range. In‐vitro studies showed that most of the DF 32 P label was intraerythrocytic, bound to the erythrocyte B esterase and not to haemoglobin. In view of these findings, it is suggested that [ 51 Cr]chromate is a valid label for studying red‐cell survival in haemoglobin H disease.