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Changes in 32 P‐Labelling of Platelet Phospholipids in Response to ADP
Author(s) -
Lloyd J. V.,
Nishizawa E. E.,
Haldar J.,
Mustard J. F.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb07092.x
Subject(s) - phosphatidic acid , platelet , labelling , phospholipid , phosphatidylinositol , chemistry , chromatography , phosphate , biochemistry , membrane , adenosine diphosphate , thin layer chromatography , biology , platelet aggregation , immunology , kinase
S ummary . Suspensions of washed platelets from rabbits were labelled in vitro with 32 PO 4 , washed, and then stimulated with ADP. Thirty seconds after the addition of ADP or of a control solution, samples of the suspension were subjected to lipid extraction. The phospholipid classes were isolated by thin layer chromatography. The results of phosphate estimation on the isolated phospholipids were in agreement with the previously described amounts of diphosphatidylinositol (DPI) and triphosphatidylinositol (TPI) in human platelets. In both ADP‐treated and control plateletes the label in phospholipid was mainly in phosphatidic acid (PA), phosphatidylinositol (MPI), DPI and TPI. Thirty seconds after the addition of ADP there was an increase in labelling of PA, DPI and TPI but the amounts of PA, DPI and TPI in platelets did not change. The results indicate that ADP probably induces an increase in turnover of the phosphate moieties of these phospholipids. The changes observed represent changes in the metabolism of the phospholipids of the platelet membrane which may be important in the mechanism of platelet aggregation.