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The Metabolism of Maturing Reticulocytes
Author(s) -
Gasko O.,
Da D.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb07088.x
Subject(s) - citric acid cycle , metabolism , biochemistry , chemistry , metabolic pathway , respiration , oxygen , glycolysis , tricarboxylic acid , mitochondrion , cellular respiration , succinate dehydrogenase , biology , organic chemistry , botany
S ummary A functional Krebs tricarboxylic acid cycle was demonstrated to exist in rabbit reticulocytes by the following methods:1 Measurement of the rate of oxygen consumption of the cells, using acetate and succinate as substrates. 2 Demonstration of the Crabtree effect exerted by glucose on cellular respiration. 3 Following the metabolic pathway of [2‐14 C]acetate, [3‐14 C]pyruvate and [6‐14 C]glucose and measuring the formation of labelled CO2 by the cells. The effects of some inhibitors of mitochondrial activity on this pathway were also studied. 4 Demonstration of the conversion of labelled acetate into various intermediates of the Krebs cycle by chromatography and autoradiography.