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Comparative Effects of Fibrinogen Degradation Fragments D and E on Coagulation
Author(s) -
Larrieu M. J.,
Rigollot C.,
Marder V. J.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb05717.x
Subject(s) - fibrinogen , thrombin , fibrin , chemistry , coagulation , biochemistry , cleavage (geology) , platelet , stereochemistry , biology , immunology , medicine , paleontology , fracture (geology)
S ummary The mechanism of anticoagulant action of the fibrinogen degradation products (fragments D and E) has been investigated using purified products in selected esterolytic, proteolytic and coagulation systems. Their modes of action were distinctly different. Fragment D acted primarily by inhibiting fibrin monomer polymerization, while fragment E did not significantly alter this process. The latter product appeared to be a competitive inhibitor of the clotting action of thrombin on fibrinogen. Its comparative effect in thrombin proteolytic and esterolytic systems indicated that fragment E did not irreversibly inactivate the enzyme centre of thrombin. Rather, it appeared to block a thrombin binding site essential for proper alignment with the fibrinogen substrate. This competitive inhibition suggests that fragment E may derive from and share physical structures with the thrombin‐susceptible, N‐terminal portion of the fibrinogen molecule.