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Co‐ordinated Increase of Sodium Leak and Sodium Pump in Hereditary Spherocytosis
Author(s) -
Wiley J. S.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb05700.x
Subject(s) - hereditary spherocytosis , ouabain , sodium , chemistry , medicine , endocrinology , leak , microbiology and biotechnology , biochemistry , biology , organic chemistry , environmental engineering , engineering
S ummary The presence of an increased sodium leak into hereditary spherocytes up to three times normal has been confirmed from measurement of 22 Na + influx into red cells. 22 Na + influx was not identical to the net inward leak of Na + but the two kept a constant relationship since influx was about double the net Na + gain in both normal and abnormal cells incubated with ouabain. The intracellular concentration of Na + ions in fresh cells from hereditary spherocytes was identical to that in normal red cells. Hereditary spherocytes showed increased maximal activity of a membrane ATP‐ase inhibited by ouabain and the Na + concentration giving half‐maximal activation of this ATP‐ase was the same for spherocytes and normal cells. When the 22 Na + influx into red cells from nine patients with hereditary spherocytosis was compared with maximal activity of cation pump ATP‐ase in the same cells a significant correlation was obtained ( r = 0.78, P > 0.01). The results indicate some linkage between the increase in the Na + leak and functional number of active cation pumps in the membrane of hereditary spherocytes.