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Two Progressive Inhibitors of Factor Xa in Human Blood
Author(s) -
Marciniak Ewa,
Tsukamura Sumiko
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb05680.x
Subject(s) - antithrombin , chemistry , sephadex , size exclusion chromatography , biochemistry , in vivo , heparin , human plasma , chromatography , microbiology and biotechnology , enzyme , biology
S ummary The limited ability of human blood to inactivate human factor Xa was found to be markedly increased in human plasma and serum intensively absorbed by aluminium hydroxide. Using gel‐filtration analysis on Sephadex G‐200 it was demonstrated that the activity brought about by adsorption resides in the macro‐globulin fraction. This fraction, as obtained from nonabsorbed plasma or serum, has low antifactor‐Xa activity; it represents a progressive inhibitor of this enzyme, additional to antithrombin III. The increase of inhibitory activity due to adsorption is probably based on removal of a substance associated with macroglobulin in vivo , and on uncovering new binding sites for factor Xa. Whereas antithrombin III and the antifactor Xa of lower molecular size are probably represented by a single protease inhibitor which may be potentiated by heparin, the macroglobular anti‐factor Xa is not closely related to antithrombin activity and is unaffected by heparin.