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Experimental Transfusion Reactions in Monkeys: Haemolytic, Coagulant and Renal Effects of Transfused Isoimmune IgG and IgM
Author(s) -
Lopas H.,
Birndorf N. I.,
Bell C. E.,
Robboy S. J.,
Fortwengler H. P.,
Biddison W. E.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb03491.x
Subject(s) - haemolysis , immunology , antibody , anuria , hemolysis , fibrin , immune system , medicine , coagulation , fibrinogen , platelet , immunoglobulin g , oliguria , disseminated intravascular coagulation , immunoglobulin m , renal function
S ummary . IgG and IgM fractions were purified from plasmas of six pairs of monkeys cross‐immunized with each others' red cells. Essentially all haemolytic activity was found in IgG fractions. Immune IgG or IgM was transfused into incompatible recipients. A dose of immunoglobulin equivalent to that present in 10 ml of plasma per kg of recipient body weight was used. Red cell mass decreased in direct proportion to quantitative haemolysin content of IgG during the first 4 hr after transfusion. A small decrease was also noted after one IgM transfusion. Parallel increases in plasma and urinary haemoglobin were observed. Disseminated intravascular coagulation (DIC) and anuria or oliguria were seen after every immune IgG infusion. DIC, characterized by falls in platelet count, fibrinogen content, levels of factors V and VIII, and fibrin clots at autopsy varied in severity with the extent of the haemolysis. The amount of haemolysis in vivo was accurately predictable by the QH 50 , a sensitive in vitro assay of antibody haemolytic activity. No coagulation changes or decreases in urinary volume were noted after immune IgM or control immunoglobulin infusions. These immune IgG infusions in monkeys provide a model for the study of the pathophysiology and therapy of human transfusion reactions.