Premium
Selective in Vitro Cytotoxicity of a Glycolytic Enzyme Inhibitor for Immature Lymphoid Cells
Author(s) -
Mann J. R.,
Simpson J. S.,
McEvoy M. W.,
Stuart J.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb03462.x
Subject(s) - lactate dehydrogenase , cytotoxicity , lymphoblast , glycolysis , in vitro , biochemistry , cytotoxic t cell , microbiology and biotechnology , enzyme , chemistry , phagocytosis , myeloid , biology , cell culture , immunology , genetics
S ummary . A specific lactate dehydrogenase inhibitor, sodium oxamate, which prevents glycolytic resynthesis of ATP, has been shown to possess a significant, in vitro cytotoxic effect for EB 2 cells and for the acute leukaemia lymphoblast. This result was obtained at an oxamate concentration of 0.08 M in TC 199 medium adjusted to physiological osmolality, in comparison with control cultures containing an equimolar concentration of sodium chloride. No significant oxamate cytotoxicity was found for normal marrow myeloid precursors or for blood lymphocytes and neutrophils, although a significant reduction in glycolysis‐dependent neutrophil phagocytosis was obtained.