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Leucocyte Response to Bacterial Endotoxin in Mice Pre‐treated with Cytotoxic Agents
Author(s) -
Twentyman P. R.
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb03460.x
Subject(s) - cytotoxic t cell , peripheral blood mononuclear cell , methotrexate , immunology , bone marrow , tail vein , peripheral blood , pharmacology , medicine , andrology , biology , in vitro , biochemistry , microbiology and biotechnology , in vivo
S ummary . The leucocyte response to bacterial endotoxin has been observed in normal mice and in mice which had been treated, either 5 or 9 days previously, with dimethyl myleran (DMM) or methotrexate (MTX). Following administration of endotoxin, the number of polymorphonuclear leucocytes in tail vein blood rose by a factor of 7 over a period of 8–12 hr, and subsequently declined slowly. Mononuclear leucocytes, on the other hand, fell by a factor of 2 within 2 hr and then slowly recovered. The response patterns in mice pretreated with the cytotoxic agents were little changed, despite a considerably lower initial cell count in animals which had received dimethyl myleran. Such a tendency towards a constant ‘mobilization ratio’ of polymorphonuclear cells must reduce the value of the endotoxin response test as a method of examining the status of marrow reserves.