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Blood Clotting Activity of Reptilase and Bovine Thrombin in Vitro: A Comparative Study on Seven Different Species
Author(s) -
Wik K. Ove,
Tangen Oddvar,
Mckenzie F. Neil
Publication year - 1972
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1972.tb03457.x
Subject(s) - thrombin , fibrinogen , chemistry , clotting time , clotting factor , platelet , in vitro , guinea pig , batroxobin , biochemistry , microbiology and biotechnology , immunology , endocrinology , biology , medicine
S ummary . The clotting activities of Reptilase and purified bovine thrombin towards platelet poor plasma from seven species and purified human and rabbit fibrinogen were studied. Bovine thrombin exhibited particularly low activity towards plasma from rat, guinea‐pig and cow compared with the other species tested. Reptilase showed a relatively larger species dependent variation in activity than thrombin, and rabbit plasma was particularly resistant to Reptilase action. This was due to a low activity of Reptilase towards rabbit fibrinogen and not to inhibition of Reptilase in rabbit plasma. The presence of 1% (w/v) dextran (70 000 mol wt) in the clotting mixtures significantly decreased both the thrombin and Reptilase clotting times in plasmas from all species.