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Assay of Megakaryocytopoiesis in Thrombocytopenic Rats
Author(s) -
Odell T. T.,
Jackson C.W.,
Friday T.J.,
Du K. Y.
Publication year - 1971
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1971.tb03434.x
Subject(s) - megakaryocytopoiesis , labelling , platelet , bone marrow , thymidine , megakaryocyte , haematopoiesis , immunology , medicine , endocrinology , andrology , microbiology and biotechnology , chemistry , biology , biochemistry , dna , stem cell
S ummary . Rats were given exchange transfusions with different volumes of platelet‐poor blood, injected 24 hr later with tritiated thymidine, and killed after another 24 or 36 hr. Autoradiograms of bone marrow smears were made and the labelling index of megakaryocytes was determined. The labelling index was proportional to the degree of thrombocytopenia induced by exchange transfusion. The results therefore demonstrate that the number of circulating platelets exerts a rather delicate control over megakaryocytopoiesis. In addition, the potential of this procedure for studying megakaryocytopoiesis, its regulation, and its abnormalities is indicated.