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Factor X in Freeze‐dried Factor‐VIII Reference Plasma
Author(s) -
Brozović M.,
Gurd L. J.,
Robertson I.,
Bangham D. R.
Publication year - 1971
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1971.tb03430.x
Subject(s) - chemistry , chromatography
S ummary . The activity and stability of factors X and VIII were studied in three ampouled preparations of freeze‐dried pooled human plasma. Factor‐X activity in all three preparations decreased on storage. It was calculated that after 10 yr at – 20°C, between 3% and 8% of the original factor‐X activity would be lost. If stored for the same period at 4°C and 20°C the loss could be up to 54% and 90% respectively of the initial activity. If, however, factor VIII was added to restore the concentration of this factor to approximately normal level, the proportion of factor X available for activation via the intrinsic pathway remained the same irrespective of the temperature and length of storage. Factor‐VIII activity was lost more readily. It was estimated that between 20% and 30% of the activity would be lost in each of the preparations kept at ‐ 20°C in 10 years. This is in good agreement with the results of the international collaborative study carried out to establish the international standard for factor VIII.