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Haemolysis and Intravascular Coagulation due to Incompatible Red Cell Transfusion in Isoimmunized Monkeys
Author(s) -
Lopas H.,
Birndorf N. I.
Publication year - 1971
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1971.tb02700.x
Subject(s) - haemolysis , medicine , red cell , pure red cell aplasia , disseminated intravascular coagulation , anuria , coagulation , hemolysin , oliguria , antibody , immunology , pathology , surgery , chemistry , biochemistry , bone marrow , virulence , renal function , gene
S ummary . Ten randomly selected cynomolgus monkeys were each given an intramuscular injection of a mixture of isologous red cells suspended in complete Freund's adjuvant. After 6 weeks a second injection of red cells in incomplete Freund's was given and thereafter intravenous booster injections of red cells in isotonic saline were used. Antibody titres were determined with serial crossmatches at 37°C, 4°C, and with rabbit antimonkey serum. All animals developed antibody to at least one and several against all four immunizing red cells. Haemolysins were seen frequently. Transfusion reactions followed intravenous injections of incompatible 51 Cr‐labelled red cells. Major reactions occurred in five of seven animals and consisted of complete destruction of infused cells within 5 min, haemoglobinuria, haemoglobinaemia, oliguria, anuria in one case, and the disseminated intravascular coagulation syndrome in three cases. The remaining two animals had mainly extravascular destruction of the transfused red cells and exhibited no coagulation changes.