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Immunological Studies of the Renal Erythropoietic Factor (Erythrogenin)
Author(s) -
McDonald Ted P.,
Zanjani Esmail D.,
Lange Robert D.,
Gordon Albert S.
Publication year - 1971
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1971.tb00791.x
Subject(s) - erythropoiesis , medicine , endocrinology , in vitro , antibody , chemistry , immunology , anemia , biochemistry
S ummary . An immunological study of the renal erythropoietic factor (REF or erythrogenin) has been described. The experiments indicate that the erythropoietic activity of REF, as assayed in the polycythaemic mouse, is neutralized in vitro by addition of serum obtained from a rabbit previously immunized with a REF preparation. These anti‐REF sera had no effect on the biological activity of ESF; however, a depression in erythropoiesis was observed after injection of anti‐REF into normal mice. It is concluded that the injection of an antibody developed against the REF into normal mice interrupts normal erythropoiesis by reducing REF levels; the reduced REF levels lead to decreased amounts of ESF.