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Factors Influencing Chromium Elution from Labelled Red Cells in Vivo and the Effect of Elution on Red‐Cell Survival Measurements
Author(s) -
Szymanski I. O.,
Valeri C. R.
Publication year - 1970
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1970.tb01636.x
Subject(s) - elution , chromium , chromatography , chemistry , in vivo , labelling , saline , dilution , radiochemistry , biochemistry , biology , endocrinology , physics , microbiology and biotechnology , organic chemistry , thermodynamics
S ummary . Rates of 51 Cr elution in vivo were studied after 32 transfusions of previously‐frozen and liquid‐preserved red cells, the survival of which was followed by measurement of the persisting radioactivity and by an automated differential agglutination (ADA) procedure. Both rapid and slow phases of chromium elution were observed; the data suggested that in‐vitro labelling conditions influenced both the quantity of rapidly‐eluting chromium and the rate of elution of the remaining label. Approximately 3% of the chromium label eluted rapidly from red cells that had been washed with phosphate‐buffered saline prior to labelling, while less than 1% of the chromium eluted rapidly from non‐washed concentrated red cells. By contrast, approximately 8.5% of the chromium label eluted rapidly from red cells washed by dilution/agglomeration, whereas approximately 11.8% of the chromium eluted rapidly from either non‐washed whole blood or from red cells washed with non‐buffered saline prior to chromium labelling. Moreover, with the latter labelling conditions, the 24 hr chromium survival value was at times considerably lower than the ADA value. The slow phase of in‐vivo elution was significantly more rapid for red cells labelled as whole blood (non‐washed) and for those washed with non‐buffered sodium chloride (mean half period of elution 44.8 days), than for red cells washed by dilution/agglomeration and for non‐washed concentrated red cells (mean half periods 74.2 and 75.3 days respectively). An intermediate slow rate of elution for red cells washed with phosphate‐buffered sodium chloride (mean half period 63.2 days) was not significantly different from slow phase elution rates observed in the other groups.