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Heterogeneity of Human Platelets III. GLYCOGEN METABOLISM IN PLATELETS OF DIFFERENT SIZES
Author(s) -
Karpatkin Simon,
Charmatz Arthur
Publication year - 1970
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1970.tb01612.x
Subject(s) - glycogenolysis , platelet , glycogen , glycogenesis , glycogen phosphorylase , medicine , endocrinology , chemistry , gluconeogenesis , glycogen synthase , fructose , thrombopoiesis , biochemistry , metabolism , biology , megakaryocyte , genetics , stem cell , haematopoiesis
S ummary Glycogenolysis, glycogenesis, glyconeogenesis and the enzymes phosphorylase, glycogen synthetase, and fructose‐1–6‐diphosphatase were investigated in heavy and light platelet populations in order to determine the presence or extent of metabolic heterogeneity. The mean volume of the heavy platelets was approximately 2.4‐fold greater than that of the light platelets. Heavy platelets contained 3.4‐fold more glycogen and had a 5.9‐fold greater rate of glycogenolysis per g wet weight, than light platelets. Heavy platelets incorporated 5.9‐fold more [ 14 C]glucose into platelet glycogen when data were expressed per g wet weight, and 1.7‐fold more [ 14 C]glucose when data were expressed per μmole of glycogen. This difference in glycogen synthesis was paralleled by a 1.8‐fold greater glycogen synthetase activity. Heavy platelets incorporated 3.0‐fold more [ 14 C]citrate into platelet glycogen when data were expressed per g wet weight, and 1.4‐fold more [ 14 C]citrate when data were expressed per μmol of glycogen. Similar results were obtained utilizing [ 14 C]pyruvate as precursor. This difference in glyconeogenesis was paralleled by a 2.5‐fold greater fructose‐1–6‐diphosphatase activity for heavy platelets.