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Effects of Thrombocytopenia on Megakaryocytopoiesis
Author(s) -
Odell T. T.,
Jackson C. W.,
Friday T. J.,
Charsha Deborah E.
Publication year - 1969
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1969.tb05667.x
Subject(s) - megakaryocytopoiesis , megakaryocyte , platelet , antiserum , mitotic index , bone marrow , mitosis , population , thymidine , immunology , andrology , labelling , biology , haematopoiesis , endocrinology , medicine , microbiology and biotechnology , antibody , biochemistry , stem cell , dna , environmental health
S ummary . Megakaryocytopoiesis was studied in rats made thrombocytopenic by daily injections of platelet‐specific antiserum. Tritiated thymidine was injected 24 hours after the first antiserum and rats were killed at intervals up to 72 hours after the thymidine. Megakaryocyte number and mitotic index were determined on sections of bone marrow; the labelling index (per cent of cells labelled) and frequency of megakaryocytes in morphologic compartments were determined in autoradiograms of marrow smears. Thrombocytopenia caused an increase in the numbers of megakaryocytes, beginning about a day after the first injection of antiserum, with young forms becoming more frequent in the recognizable population. At about the same time, there was an increase in the mitotic index (endomitosis) of megakaryocytes. All of these results indicated an increased influx of cells into the recognizable megakaryocyte population. Later the frequency of older forms increased, revealing that a cohort of cells was passing through the maturation process. The changes in labelling index suggested that megakaryocytes may mature more rapidly in thrombocytopenic rats than in controls. The results suggest that a regulatory agent may act on a diploid precursor cell in responding to the thrombocytopenic state.