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The Distribution of Folates in Rabbit Tissues during Phenylhydrazine‐Induced Haemolysis
Author(s) -
Izak G.,
Rachmilewitz M.,
Grossowicz N.,
Galewski K.,
Jablonska M.
Publication year - 1969
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1969.tb00406.x
Subject(s) - haemolysis , reticulocytosis , phenylhydrazine , in vitro , bone marrow , chemistry , in vivo , reticulocyte , red cell , hemolysis , biochemistry , biology , pharmacology , immunology , medicine , anemia , messenger rna , microbiology and biotechnology , medicinal chemistry , gene
Summary: Acute haemolysis induced in rabbits with acetylphenylhydrazine (APH) was associated with a high reticulocytosis and increased folate activity with all three bioassays ( L. casei, S. faecalis and P. cerevisiae ) employed, in comparison with untreated animals. The bone marrow folate activity of the APH‐treated rabbits was considerably higher than that of control marrows; activity measured with P. cerevisiae reached 50 per cent of that obtained with L. casei , indicating a high conversion into reduced forms. Administration of pteroylglutamate (PGA) together with APH resulted in higher red cell folate values than administration of APH alone. In vitro , reticulocytes from APH‐treated rabbits incorporated three times more radioactive‐PGA than those from animals given APH with PGA. The various aspects regulating the uptake and function of folates in red cells at varying degrees of maturation are discussed.