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Further Observations on Spontaneous Blastoid Transformation of Human Small Lymphocytes in Vitro
Author(s) -
Caron G. A.
Publication year - 1969
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1969.tb00405.x
Subject(s) - phytohaemagglutinin , blastoid , transformation (genetics) , in vitro , thymidine , biology , mixed lymphocyte reaction , immunology , medicine , microbiology and biotechnology , genetics , immune system , t cell , lymphoma , mantle cell lymphoma , gene
Summary: DNA synthesis, measured by tritiated thymidine uptake during the final 1 hour in vitro , has been used as a measure of blast transformation of human peripheral small lymphocytes cultured in media containing 20 per cent autologous plasma. Control cultures from two subjects of up to 12 and 15 days duration and from eight further subjects of 3 and 6 days duration have been studied and the degree of spontaneous transformation compared to the response to known stimulants of transformation, phytohaemagglutinin and specific antigens. Spontaneous transformation was consistently low for up to 6 days. Thereafter there was a relatively slight increase. Theoretical arguments why the increased proportion of labelled cells found in some of the older cultures may be an artifact of differential cell survival are presented. Review of previous reports of spontaneous lymphocyte transformation suggests that this is minimal when the cells are cultured in autologous plasma and that heterologous sera may act as an unsuspected transformation stimulant.