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A Method for the Positive Identification of Erythropoietic Cells in Chromosome Preparations of Bone Marrow
Author(s) -
Rastrick Joan M.
Publication year - 1969
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1969.tb00392.x
Subject(s) - bone marrow , ascorbic acid , chromosome , myeloid , formaldehyde , chemistry , erythropoietin , cell culture , biochemistry , biology , microbiology and biotechnology , pathology , cancer research , medicine , immunology , genetics , gene , food science
Summary: Chromosome preparations from the bone marrow of four patients with chronic granulocytic leukaemia were made after the cells had been cultured for 12 hours with iron isotopes in a medium with added GSH, ATP, cysteine and ascorbic acid, which permitted optimal iron uptake and incorporation. The cells were fixed for 15 minutes only in formaldehyde‐methanol‐acetic acid. Auto‐radiographs of these preparations showed that the Ph1 chromosome was present in erythroid cells. This indicates that erythroid and myeloid cell lines have a common stem cell in the bone marrow.