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Erythropoietin and Haemoglobin Studies on AKR Mice
Author(s) -
Lewis Jasper P.,
Alford Dorothy A.,
Smith James E.,
Horton Bennett F.,
Smith Linda L.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb06997.x
Subject(s) - erythropoietin , chemistry , hemoglobin , sephadex , glutathione , biochemistry , elution , chromatography , endocrinology , biology , enzyme
S ummary . DEAE‐Sephadex column chromatography has been used to study the haemoglobins of AKR untreated mice, polycythaemic mice injected with saline and polycythaemic mice treated with an extract of human erythropoietin. All elution patterns of the haemolysates showed four haemoglobins. The haemolysates of each group had an elution pattern different from the other groups. Chromatographic studies of an ageing haemolysate indicated the formation of a minor haemoglobin from a major haemoglobin to be linear for a period of about 18 days. The major AKR mouse haemoglobin appeared to polymerize, possibly to octaniers. Human erythropoietin, when injected into polycythaemic mice, seemed to decrease the resulting polymerization of the major haemoglobin and the formation of the minor haemoglobin. Treatment of the haemolysates with reduced glutathione seemed to depolymerize the polymers to tetramers. An extract of human erythropoietin lowered the glutathione level in the blood of an AKR strain of mice.