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The in vitro Effect of Phytohaemagglutinin on Separated Human Bone Marrow Cells
Author(s) -
Pegrum G. D.,
Ready Doreen,
Thompson Elizabeth
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01553.x
Subject(s) - phytohaemagglutinin , uridine , thymidine , in vitro , rna , microbiology and biotechnology , dna synthesis , dna , bone marrow , biology , biochemistry , tissue culture , chemistry , immunology , gene
Cells from 60 marrow aspirates were separated using columns of glass microspherules. The effect of PHA on the viability and synthesis of DNA and RNA by these cells was observed after 3 days growth. The synthesis of DNA and RNA was observed before and after culture by means of autoradiographs prepared after exposure to 3 H thymidine or 3 H uridine. 3 H thymidine uptake was low in the initial suspensions and was not increased by the addition of PHA, 3 H uridine uptake was increased in only four of 41 cultures stimulated with PHA, and there was a reduction in the number of viable cells in cultures containing PHA.