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Immunological Studies of Proteins Associated with the Subcellular Fractions of Thrombasthenic and Afibrinogenaemic Platelets
Author(s) -
Nachman R. L.,
Marcus A. J.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01528.x
Subject(s) - platelet , fibrinogen , thrombasthenia , chemistry , biochemistry , intracellular , microbiology and biotechnology , biology , immunology , platelet aggregation
Immunological studies of proteins associated with subcellular fractions of normal, thrombasthenic, and afibrinogenaemic platelets are reported. Thrombosthenin, the contractile protein of platelets, was present in afibrinogenaemic and thrombasthenic platelets in approximately normal amounts as determined by fluorescent antibody staining. Thrombasthenic and afibrinogenaemic platelets were markedly deficient in cell‐bound fibrinogen. One hundred per cent of afibrinogenaemic platelet fibrinogen was present in the intracellular granule fraction compared to 10 per cent in thrombasthenic and 27 per cent in normal platelets. An abnormal protein pattern was observed in the thrombasthenic granule preparation, while proteins derived from thrombasthenic membranes were deficient in one of the normal membrane components. The soluble ‘cell sap’fractions of the afibrinogenaemic and thrombasthenic platelets contained normal amounts of albumin, γ‐globulin and Factor XIII. The results suggest that decreased platelet fibrinogen in thrombasthenia may be a secondary phenomenon, perhaps related to an underlying membrane defect. The presence of platelet fibrinogen in the granule fraction of afibrinogenaemic platelets supports the concept of two separate pools of fibrinogen—intracellular and extracellular.