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The Metabolism of Phytohaemagglutinin during Incubation with Human Peripheral Lymphocytes
Author(s) -
Naspitz Charles K.,
Richter Maxwell
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01513.x
Subject(s) - phytohaemagglutinin , incubation , incubation period , in vitro , mitogen activated protein kinase , biology , mitosis , metabolism , immunology , phytohemagglutinins , andrology , endocrinology , microbiology and biotechnology , biochemistry , immune system , lymphocyte activation , medicine , t cell
The mechanism of action of PHA in in vitro leucocyte cultures was investigated with respect to the fate of the mitogen during the 3‐day blastogenic response. It was demonstrated that incubation of the leucocytes with PHA for only 5 minutes followed by incubation for 72 hours in medium devoid of PHA was sufficient to initiate mitosis and blastogenesis. However, the minimum incubation time with PHA required to induce maximum blastogenesis was 6 hours. Furthermore, it was demonstrated that the mitogen is bound to cells in culture but the experimental findings suggest that this cell‐bound mitogen is not degraded during the 3‐day incubation period.