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Infusion of Marrow and Spleen Cells in Irradiated NZB/Bl Mice: Amelioration of Autoimmune Disease
Author(s) -
Lindsey Edward S.,
Woodruff M. F. A.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01495.x
Subject(s) - spleen , bone marrow , haemolysis , coombs test , immunology , medicine , antibody , pathology , andrology
S ummary Adult NZB/Bl mice which showed a positive Coombs' test and other evidence of abnormal haemolysis were exposed to 800 r. whole body irradiation. They were then given an intravenous injection of bone marrow or a mixture of bone marrow and spleen cells from either young (Coombs negative) NZB/Bl mice or Coombs negative (NZB/Bl × T6) F 1 hybrids. One of the 14 mice which received isogeneic cells died before being retested. Nine of the remaining 13 became Coombs negative; five of these remained negative and four reverted to Coombs positive 1–8 months after cell injection. Four of the II mice which received F 1 cells died before being retested; six of the remaining seven became Coombs negative and remained so until they died or were killed 1–12 months after cell injection. Chromosome studies in the animal which survived longest in this group revealed that all dividing cells in the spleen and lymph nodes, and 90 per cent of the dividing cells in the bone marrow, were of donor origin.