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Post‐Transfusion Survival of Red Cells Stored in Liquid Nitrogen
Author(s) -
Morrison F. S.,
Mollison P. L.,
Robson D. C.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01488.x
Subject(s) - liquid nitrogen , fresh frozen plasma , nitrogen , chemistry , andrology , blood preservation , chromatography , zoology , medicine , biology , immunology , platelet , organic chemistry
S ummary In 13 cases in which red cells were stored in a PVP‐ACD mixture at 4°C. for not more than 24 hours before being frozen in liquid nitrogen and were then stored in liquid nitrogen vapour (−150°C.) for periods up to 486 days, post‐transfusion 51 Cr survival at 24 hours varied from 57 to 83.5 per cent with a mean of 69.8 per cent. There was no evidence that the period of storage at −150°C. had any influence on survival. On the other hand, minor variations in the method of labelling the cells with 51 Cr did appear to affect the results; there was some evidence that repeated washing of the cells in PVP‐plasma had an unfavourable effect. In a further 14 cases in which red cells were stored in PVP‐plasma at 4°C. for up to 48 hours before freezing and for 1–32 days after thawing, evidence was obtained first, that storage for more than 24 hours before freezing was deleterious and, second, that storage for up to 12 days probably had no adverse effects and, thirdly, that storage for 15 days or more after thawing was very deleterious. In two experiments in which cells were labelled both with DF 32 P and with 51 Cr it was found that Cr did not elute at an abnormal rate from red cells which had been frozen and thawed after being mixed with PVP. Accordingly, standard corrections for Cr elution may be applied and it may be concluded that the true 24‐hour survival of red cells frozen and thawed in liquid nitrogen averages about 73.5 per cent.