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Relationship between Membrane Function and Permeability: III. FURTHER EVIDENCE LINKING MEMBRANE TRANSPORT AND THROMBOPLASTIN AVAILABILITY OF THE INTACT ERYTHROCYTE
Author(s) -
Cohen Phin
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb01483.x
Subject(s) - erythritol , glycerol , ethylene glycol , membrane , chemistry , biochemistry , thromboplastin , permeability (electromagnetism) , biophysics , chromatography , organic chemistry , biology , medicine , coagulation
S ummary Within a series of short straight‐chain compounds resembling glycerol in structure, the pattern of unmasking of erythrocyte ‘platelet factor 3’ (PF‐3) activity appeared to be related to a combination of cell penetrability, molecular weight and complement of hydroxyl groups. The same relationship held in a series of low molecular weight polymers of ethylene glycol. Moreover, propylene glycol and ethylene glycol monomethylether, at very low concentrations, were shown to delay glycerol‐induced erythrocyte PF‐3 enhancement. Among penetrating non‐electrolytes not having a structural similarity to glycerol, dimethylsulphoxide was the only compound that had a family of PF‐3 enhancement curves of the glycerol type. The glycerol induced PF‐3 enhancement curves with cow and sheep erythrocytes were similar to those of erythritol with human erythrocytes. Furthermore, the attainment of equilibrium of 14 C erythritol between human erythrocyte and plasma compartments correlated temporally with unmasking of PF‐3 activity by erythritol solutions. The discussion attempts to relate these findings to new theoretical views which emphasize a close link between membrane transport and thromboplastin availability in the intact erythrocyte.