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Changes in Erythrocyte Membrane and Autohaemolysis during in Vitro Incubation
Author(s) -
Langley G. R.,
Axell M.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb00365.x
Subject(s) - incubation , phosphatidylethanolamine , phosphatidylserine , phospholipid , sphingomyelin , lecithin , incubation period , cholesterol , biochemistry , biology , membrane , chemistry , phosphatidylcholine
S ummary . Spontaneous autohaemolysis occurs as sterile defibrinated blood is incubated at 37°C. This study was undertaken to identify the membrane changes which occurred during autohaemolysis and the effect upon these of supplementary glucose. After 24 hours of incubation the earliest membrane change recognized was the loss of small amounts of cholesterol; this occurred equally in incubations with and without added glucose. Spherocytes were prominent in incubations without added glucose but were infrequent when supplementary glucose was provided. During the second 24 hours of incubation cholesterol loss increased and phospholipids eluted from the cell surface. In this period of incubation cholesterol and phospholipid loss was less in incubations provided with supplementary glucose. The major individual phosphatides lecithin, sphingomyelin, phosphatidylethanolamine and phosphatidylserine were lost in proportion to their original concentration in the membrane. Membrane protein did not decrease as cell lipid loss occurred.