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The Effect of Collagen on Platelet Glycolysis and Nucleotide Metabolism
Author(s) -
Loder P. Bronwen,
Hirsh J.,
Gruchy G. C.
Publication year - 1968
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1968.tb00363.x
Subject(s) - glycolysis , platelet , nucleotide , adenine nucleotide , chemistry , metabolism , biochemistry , medicine , endocrinology , biology , gene
S ummary . Glycolytic activity and nucleotide metabolism were studied in normal resting platelets and platelets exposed to collagen. Lactate production was found to be a more sensitive index of glycolytic activity than glucose consumption. In the presence of added glucose, the rate of lactate production was linear for at least 60 minutes. In the absence of glucose, lactate production continued for 60 minutes and appeared to be independent of glucose for the first 10–15 minutes. Addition of both collagen and pure tropocollagen to the platelet suspension produced a significant and constant increase in glycolytic rate. This was associated with release of ATP and ADP and a small but significant increase in total ATP. Addition of ADP in concentrations similar to those released by collagen had no effect on glycolytic activity. Addition of calcium ions produced release of nucleotides but no increase in glycolytic rate. EDTA inhibited glycolysis both in the resting platelets and in the presence of collagen. It is suggested that the stimulation of glycolysis by collagen may be due to the action of collagen on one or more rate‐limiting glycolytic enzymes.