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Sequence of Events Terminating in Death of Leucocytes
Author(s) -
Aune Janet M.,
Gantner G. E.,
Wade N. J.
Publication year - 1967
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1967.tb08852.x
Subject(s) - incubation , glycogen , intracellular , programmed cell death , cytoplasm , motility , biology , cell injury , cellular metabolism , microbiology and biotechnology , cell damage , biochemistry , carbohydrate metabolism , metabolism , endocrinology , apoptosis
SUMMARY Cellular damage in human granulocytes was evident by light microscopy after 24 hours of 30° C. incubation in unfortified whole blood. Injury was indicated by the cell's inability to accommodate neutral red and the simultaneous loss of motility. Cellular death was delimited to this loss of physiological integrity. Biochemical changes which occurred intracellularly during incubation were correlated with changes in the surrounding milieu: plasma glucose diminished first, and after this was depleted there was histochemical evidence of decreased cytoplasmic glycogen. It appears by analogy, therefore, that after the glucose was utilized, intracellular glycogen may have been used for energy metabolism enabling the cell to function an additional 10–15 hours. When nutrient deficiency produced structural disintegration, the cell was unable to maintain itself and death followed.