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Antibody to Intrinsic‐Factor‐Vitamin‐B 12 Complex in Pernicious Anaemia
Author(s) -
Cooper B. A.,
Yates T.
Publication year - 1967
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1967.tb08834.x
Subject(s) - intrinsic factor , antibody , pernicious anaemia , antigen , chemistry , antigen antibody complex , dissociation (chemistry) , immunology , biochemistry , biology , vitamin b12
SUMMARY Serum from some patients with pernicious anaemia contains antibody activity to human intrinsic factor‐ 57 CoB 12 complex (IF‐ 57 CoB 12 ) which can be detected by co‐precipitation of IF‐ 57 CoB 12 ) with the globulin fraction precipitated with 45 per cent ethanol. The characteristics of this antigen‐antibody reaction include some dissociation of antigen from antibody during precipitation, and ready cross‐reaction of antibody with IF to which vitamin B 12 is not bound. The IF‐B 12 antigen also cross‐reacts with anti‐intrinsic‐factor antibody, as measured by the charcoal adsorption technique. This co‐precipitation technique may be to determine whether small quantities of B 12 ‐binding material have the immunological characteristics of intrinsic factor. Although the anti‐IF antibody present in the blood of many patients with pernicious anaemia may be responsible for this anti‐IF‐B 12 antibody activity, it is possible that a separate antibody system exists which is directed against IF‐B 12 .