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Diffusion Chamber Studies of Rat Peripheral Leucocytes in the Presence of Phytohaemagglutinin
Author(s) -
Johnson L. Ione,
Sullivan Patricia A.,
Siegel Charles D.,
Chan PoCheun,
Gordon Albert S.
Publication year - 1967
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1967.tb08727.x
Subject(s) - phytohaemagglutinin , diffusion , in vivo , peripheral , consistency (knowledge bases) , immunology , biology , peripheral blood , microbiology and biotechnology , chemistry , medicine , genetics , computer science , physics , artificial intelligence , thermodynamics
SUMMARY Rat peripheral leucocytes have been cultured in diffusion chambers in an effort to achieve greater consistency in the response of lymphocytes to phytohaemagglutinin. The diffusion chamber was found to provide an in vivo environment which obviates drastic changes in pH and eliminates other factors that act as major deterrents to successful and optimal responses of lymphocytes to this mitogen in vifro. The system shows reliability and consistency in maintaining excellent cytologic and morphologic integrity of the cells in culture.