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Spontaneous Lymphocyte Transformation in vitro: Negative Evidence from a Study of Thymidine Uptake
Author(s) -
Caron G. A.
Publication year - 1967
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1967.tb08695.x
Subject(s) - thymidine , blastoid , in vitro , lymphocyte , stimulation , transformation (genetics) , peripheral blood , tissue culture , biology , microbiology and biotechnology , chemistry , immunology , biochemistry , endocrinology , lymphoma , mantle cell lymphoma , gene
SUMMARY Studies of the uptake of tritiated thymidine by human peripheral lymphocytes cultured in autologous plasma or, after washing, in foetal calf serum have failed to provide evidence for the occurrence of spontaneous blastoid transformation during 1–7 days in vitro. Repeated washing of peripheral lymphocytes caused an inconstant reduction in their ability to respond to stimulation with PHA. The accuracy and sensitivity of the technique of measuring the degree of in vitro transformation activity of lymphocyte cultures by their uptake of radioactively labelled thymidine is increased by partially removing polymorphonuclear leucocytes from the leucocyte suspensions and by restricting exposure of the cultures to the radioactive thymidine to the final 1 hour of culture.