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Kinetics of Rapidly Labelled RNA in Human Leukaemia Cells
Author(s) -
Sinks L. F.,
Hayhoe F. G. J.
Publication year - 1966
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1966.tb00163.x
Subject(s) - rna , cytoplasm , phytohaemagglutinin , nucleolus , biology , nucleus , cell , hela , cell nucleus , microbiology and biotechnology , intracellular , biochemistry , in vitro , gene
T he kinetics of rapidly labelled RNA have been studied autoradiographically in several mammalian cell types, including, among cells of human origin, Hela cells (Feinendegen, Bond, Shreeve and Painter, 1960), PHA (phytohaemagglutinin)‐induced blast cells (Hayhoe and Quaglino, 1965) and various mononuclear cells of peripheral blood (Storti and Torelli, 1965). Early work with labelled RNA precursors in leukaemic cells (Gavosto, Maraini and Pileri, 1960) showed that uptake was much diminished as compared with that of normal immature cells of the haemopoietic system, and later chase studies of movement of label from nucleus to cytoplasm, while confirming the generally low percentage of uptake, suggested that the nuclear‐cytoplasmic shift was sometimes delayed (Quaglino and Hayhoe, 1965). Again, Storti and Torelli (1965) have presented evidence that in at least some cases of acute leukaemia the labile, rapidly degraded, fraction of RNA, which may well have messenger function, is either absent or metabolically different from that of normal leucoblasts. It is clearly of great importance to explore the patterns of RNA kinetics in leukaemic cells and to determine whether the time sequence of movement of label from nucleus and nucleolus to cytoplasm and the types of RNA formed do in fact differ substantially from those of normal haemopoietic precursors or such a model cell system as is provided by PHA‐induced blast cells. As a first step we have therefore carried out a series of chase experiments in ten leukaemic cases to study the intracellular movement of a potential RNA label and to establish whether a constant pattern exists.