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59 Fe Autoradiography of Bone‐Marrow Culture from Normal Subjects and Patients with Uraemia and Pernicious Anaemia *
Author(s) -
Ward H. P.,
Tauxe W. N.,
Kiely J. M.
Publication year - 1966
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1966.tb00132.x
Subject(s) - erythropoiesis , bone marrow , iron isotopes , pathology , ineffective erythropoiesis , chronic renal failure , red cell , erythropoietin , medicine , anemia , chemistry , isotope , physics , quantum mechanics
T he kinetics of iron metabolism have been extensively investigated by radioisotopic techniques. In 1961 Pollycove and Mortimer may perhaps have carried the mathematical interpretation of the plasma iron turnover and the red cell incorporation of iron to theoretical limits. However, ferrokinetic studies have been limited by incomplete knowledge of the ‘middle compartment’ of iron metabolism, the bone marrow. Autoradiography with 59 Fe offers a direct cytological approach for study of the incorporation of iron into individual normoblasts. Austoni (1956) and Lajtha and Suit (1955) made exploratory observations with this technique in vitro and, recently, Alpen and Cranmore (1959) evaluated erythropoiesis in dogs with 59 Fe autoradiography. It was the purpose of this study to evaluate 59 Fe autoradiography in suspensions of human bone marrow and to compare the rate of 59 Fe incorporation into normoblasts of normal subjects and patients with pernicious anaemia and the anaemia of chronic renal failure.