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Microarray analysis reveals increased expression of ΔNp63α in seborrhoeic keratosis
Author(s) -
Seo E.Y.,
Lee D.H.,
Lee Y.,
Cho K.H.,
Eun H.C.,
Chung J.H.
Publication year - 2012
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2011.10665.x
Subject(s) - keratosis , microarray , microarray analysis techniques , expression (computer science) , medicine , tissue microarray , seborrhoeic dermatitis , dermatology , cancer research , biology , gene expression , oncology , pathology , immunohistochemistry , genetics , computer science , gene , programming language
Summary Background Seborrhoeic keratoses (SKs) are very common benign epidermal lesions without malignant potential. Ultraviolet radiation, old age and viruses are well‐known risk factors for disease development. However, the pathomechanisms of SK are not fully understood. Objectives To detect and characterize the genes that are involved in the pathogenesis of SK. Methods We performed a gene expression study using paired lesional and nonlesional skin samples from patients with SK. Results We identified and validated 19 differentially expressed genes in SK. Of these 19 genes, we focused on p63 transcription factor, which plays a pivotal role in epidermal development by regulating its transcriptional programme. We found by immunofluorescence that the expression of ΔNp63α, the most abundantly expressed p63 isoform, was significantly increased in SK as compared with normal skin. Moreover, siRNA‐mediated knockdown of ΔNp63 led to the downregulation of 11 genes, including a member of the tensin family TNS4 . Chromatin immunoprecipitation assay revealed that TNS4 was a target gene of p63. Conclusions We identified upregulated genes in SK using genome‐wide cDNA microarray and elucidated the functional contribution of p63 to the disease transcriptome by gene‐silencing assay. Taken together, these data may provide a novel insight into the molecular basis of these benign skin lesions.