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Calcitriol treatment improves methyl aminolaevulinate‐based photodynamic therapy in human squamous cell carcinoma A431 cells
Author(s) -
Cicarma E.,
Tuorkey M.,
Juzeniene A.,
Ma LW.,
Moan J.
Publication year - 2009
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2009.09180.x
Subject(s) - norwegian , university hospital , basal cell , medicine , library science , family medicine , philosophy , computer science , linguistics
Summary Background Photodynamic therapy (PDT) using methyl aminolaevulinate (MAL) provides a new, approved method for treatment of skin cancer and its precursors. However, MAL‐based PDT is not very efficient for poorly differentiated skin carcinoma. Thus, novel strategies to enhance the PDT effect are needed. Objectives In order to improve the efficacy of MAL‐based PDT, we investigated the effect of adding calcitriol, a prodifferentiation hormone, to human squamous cell carcinoma A431 cells in vitro . Methods A short course (24 h) of calcitriol pretreatment was applied in A431 cells, and, subsequently, MAL‐induced protoporphyrin IX (PpIX) was measured. Results Calcitriol pretreatment of the cells elevated their PpIX levels. Furthermore, the cell damage after exposure to blue light was significantly higher in calcitriol‐treated cells. Increased photoinactivation correlated with higher levels of PpIX in the calcitriol‐pretreated cells. Conclusions Calcitriol enhances MAL‐based PDT in A431 cells.