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Biphasic expression of stromal cell‐derived factor‐1 during human wound healing
Author(s) -
Toksoy A.,
Müller V.,
Gillitzer R.,
Goebeler M.
Publication year - 2007
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2007.08240.x
Subject(s) - stromal cell , wound healing , stromal cell derived factor 1 , medicine , cell , cancer research , biology , immunology , inflammation , genetics , chemokine , cxcr4
Summary Background Chemokines tightly regulate the spatial and temporal infiltration of invading leucocyte subsets during wound healing. Stromal cell‐derived factor‐1 (SDF‐1/CXCL12) is a homeostatic chemokine with multiple functions; its role during cutaneous wound healing, however, needs to be explored. Objectives To elucidate expression of the multifunctional CXC chemokine SDF‐1/CXCL12 during human wound healing. Methods Skin biopsies were obtained from 14 volunteers between 1 and 21 days after incisional wounding and processed for in situ hybridization and immunohistochemistry. Results We analysed the spatial and temporal distribution of SDF‐1/CXCL12 after artificial wounding and detected a complete downregulation at both the mRNA and the protein level within the fibrous stroma that replaces the initial wound defect. However, increased levels of SDF‐1/CXCL12 were observed at the wound margins. Focusing on mediators regulating SDF‐1/CXCL12 expression in vitro we realized that both tumour necrosis factor‐α and interferon‐γ downregulated its expression in human dermal microvascular endothelial cells and fibroblasts. Conclusions Our data suggest that SDF‐1/CXCL12 is tightly regulated during wound repair. Increased expression at the wound margin may contribute to the accumulation of endothelial progenitor cells, thus accelerating neovascularization.