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Testosterone metabolism to 5 α ‐dihydrotestosterone and synthesis of sebaceous lipids is regulated by the peroxisome proliferator‐activated receptor ligand linoleic acid in human sebocytes
Author(s) -
Makrantonaki E.,
Zouboulis C.C.
Publication year - 2007
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2006.07671.x
Subject(s) - dihydrotestosterone , medicine , endocrinology , testosterone (patch) , linoleic acid , lipid metabolism , chemistry , peroxisome , androgen receptor , androgen , biology , receptor , biochemistry , fatty acid , hormone , prostate cancer , cancer
Summary Background Despite the clinical evidence that androgens stimulate sebaceous lipids, androgens in vitro have shown no similar effects. This contradiction led to the assumption that cofactors may be required for lipid regulation and peroxisome proliferator‐activated receptor (PPAR) ligands were suggested to be adequate candidates. Objectives The influence of testosterone and linoleic acid, a PPAR ligand, as single agents and in combination with of LY191704, a 5 α ‐reductase type I inhibitor, was examined on 5 α ‐dihydrotestosterone (5 α ‐DHT) synthesis and lipid content in human SZ95 sebocytes. Methods Cell proliferation and viability were measured by the 4‐methylumbelliferyl heptanoate fluorescence assay and by the Boehringer Lactate Dehydrogenase Assay kit, respectively. 5 α ‐DHT enzyme‐linked immunosorbent assay was used for the detection of 5 α ‐DHT synthesis in cell supernatants after treatment, whereas lipid production was documented by means of the Nile red lipid microassay and fluorescence microscopy. Results Testosterone promoted 5 α ‐DHT synthesis ( P < 0·001), whereas linoleic acid increased sebaceous lipids ( P < 0·001). The combination of testosterone and linoleic acid exhibited a synergistic effect on the synthesis of 5 α ‐DHT ( P < 0·01 vs. testosterone) and sebaceous lipids ( P < 0·01 vs. linoleic acid). Furthermore, LY191704 reduced 5 α ‐DHT and sebaceous lipid levels ( P < 0·01 and P < 0·001 in comparison with testosterone/linoleic acid, respectively). Cell proliferation and viability remained unchanged under treatment with all compounds tested. Conclusions These data suggest a catalytic effect of PPAR ligands on cellular testosterone activation by 5 α ‐reduction and the importance of the latter for the regulation of sebaceous lipids.