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Differentiation potential of human embryonic mesenchymal stem cells for skin‐related tissue
Author(s) -
Wu M.,
Yang L.,
Liu S.,
Li H.,
Hui N.,
Wang F.,
Liu H.
Publication year - 2006
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2006.07357.x
Subject(s) - mesenchymal stem cell , cd90 , microbiology and biotechnology , stem cell , biology , embryonic stem cell , cellular differentiation , stem cell transplantation for articular cartilage repair , adult stem cell , pathology , cd34 , medicine , biochemistry , gene
Summary Background Mesenchymal stem cells (MSC) have the capacity to differentiate into cells of connective tissue lineages, including bone, fat, cartilage and muscle, but the differentiation of embryonic MSC into epidermal cells by mesenchymal‐epithelial transition has not been confirmed. Objectives To determine the biological characteristics of human embryonic MSC (hMSC) and their potential for differentiation into epithelial cells. Methods hMSC were derived from 4–7‐week‐old embryos; they were localized and isolated, then primary culture was done. The biological characteristics of hMSC were detected by immunohistochemical methods and flow cytometry. Their differentiation potential was determined by coculture with conditioning medium and in vivo injection. Results hMSC express the relative specific antigens of MSC, such as SH2, α ‐smooth actin, CD29, CD44, CD90 and S100. After stimulation and in vivo transplantation, hMSC possess the potential to differentiate into epidermal cells with the production of keratin 19 and E‐cadherin. Conclusions hMSC derived from the early human embryo have the ability to transform into epidermal cells in vivo and in vitro .